ABSTRACT
Introduction: establish an association between the radiographic classification and the surgical technique related to the lower third molars. Materials and method: a retrospective study was carried out, with the descriptive analysis of the data. The sample consisted of 100 patients (n = 100) from the spontaneous demand who sought out the Dental Clinic of UFC (Ceará's Federal University) Campus Sobral, between december 2017 to july 2018, and who followed the inclusion criteria: patient with panoramic radiography, free of active periodontal disease and tooth with at least two thirds of root formation; and as exclusion criteria: patients with associated bone pathologies, teeth with less than two thirds of root formation and patients who did not wish to participate in the study. Results: patients included in the study were from 18 to 25 years of age, most of them male, with complaints of pain. Using the radiographic classification of Pell & Gregory, the most found positions were 1A (35%), 2B (28%) and 2A (17%). The most prevalent surgical techniques were the open ones. Could be done the extraction by closed surgical technique (forceps or lever) (n = 27), with flap preparation plus osteotomy (OST) (n = 25) and flap plus osteotomy plus odontostomy (ODS) (n = 48). Conclusion: teeth with radiographic classification, grade of inclusion and different preoperative plans had the same protocol (flap + OST + ODS) in the surgical act. It is evident that radiographic classification stills an effective method to aid in operative planning, but it can be complemented by other diagnostic standards, such as a specific classification of the root anatomy. (AU)
Objetivo: realizar uma associação entre a classificação radiográfica e a técnica cirúrgica relacionada aos terceiros molares inferiores. Materiais e método: foi realizado um estudo retrospectivo, com a análise descritiva dos dados. A amostra foi constituída de 100 pacientes (n = 100), provenientes de demanda espontânea, que procuraram o ambulatório de Odontologia da Universidade Federal do Ceará (UFC) Campus Sobral, no período de dezembro de 2017 a julho de 2018, e que seguiram os seguintes critérios de inclusão: presença de radiografia panorâmica, ausência de doença periodontal ativa e dentes com no mínimo dois terços de formação radicular. Já os critérios de exclusão foram: pacientes com patologias ósseas associadas aos terceiros molares inferiores, dentes com menos de dois terços de formação radicular e pacientes que não desejassem participar do estudo. Resultados: os pacientes incluídos no estudo tinham idades entre 18 e 25 anos, a maioria do sexo masculino, com queixas álgicas. Em relação à classificação de Pell & Gregory, as posições mais encontradas foram 1A (35%), 2B (28%) e 2A (17%). As técnicas cirúrgicas mais prevalentes foram as abertas (73%). Em relação às técnicas cirúrgicas, foram encontradas: técnica cirúrgica fechada (fórceps ou alavanca) (n = 27), com confecção de retalho mais osteotomia (OST) (n = 25) e por retalho mais osteotomia e odontossecção (ODS) (n = 48). Conclusão: é evidente que a classificação radiográfica ainda é um método eficaz para auxiliar no planejamento operatório, mas pode ser complementada por outros padrões de diagnóstico, como uma classificação específica da anatomia radicular. (AU)
Subject(s)
Humans , Male , Female , Adolescent , Adult , Tooth Extraction/methods , Radiography, Panoramic/classification , Molar, Third/surgery , Molar, Third/diagnostic imaging , Reference Values , Tooth Root/surgery , Tooth Root/diagnostic imaging , Retrospective StudiesABSTRACT
Objetivo: detalhar indicações, vantagens e desvantagens da técnica de osteotomia pela divisão alveolar. Material e métodos: busca nos bancos de dados PubMed e Bireme, no período de 2005-2016, utilizando as palavras-chaves "atrophic maxilla", "osteotomy by alveolar division" e "oral implants", nos idiomas inglês e português, tendo como critérios de inclusão estudos de revisão da literatura, relato de caso clínico, estudos prospectivos e revisões sistemáticas. Resultados: foram encontrados 19 artigos, sendo nove estudos clínicos prospectivos, seis relatos de caso, uma análise de múltiplos casos, duas revisões da literatura e uma revisão sistemática. A técnica de osteotomia pela divisão alveolar é considerada uma alternativa viável para o processo de expansão óssea de maxilares atróficos, tornando possível o processo de reabilitação oral em pacientes com edentulismo. Conclusão: é necessário conhecer a viabilidade da técnica de osteotomia alveolar para se obter um bom resultado funcional e estético.
Objective: to discuss the indications, advantages, and drawbacks of the alveolar split osteotomy technique. Material and methods: an electronic search as wade at the PubMed and Bireme databases within 2005 and 2016 using the keywords "atrophic maxilla", "osteotomy by alveolar division", and "oral implants" at the English and Portuguese languages, including literature reviews, case reports, prospective studies, and systematic reviews. Results: 19 articles were retrieved, been 9 prospective studies, 6 case reports, 1 multiple case report, 2 literature reviews, and 1 systematic review. The osteotomy alveolar division technique is considered a viable alternative to expand the atrophic maxillae aiming the oral rehabilitation for completely edentulous patients. Conclusion: although successful, clinicians are advised to know its technical characteristics to generate a good functional and esthetic result.
Subject(s)
Humans , Dental Implants , Jaw, Edentulous , Maxilla/abnormalities , Mouth Rehabilitation , Osteotomy/methodsABSTRACT
Purpose To analyze a possible correlation between a miRNA expression profile and important prognostic factors for pTa urothelial carcinomas (UC), including tumor size, multiplicity and episodes of recurrence. Materials and Methods Thirty low-grade non-invasive pTa bladder UC from patients submitted to transurethral resection were studied, in a mean follow-up of 17.7 months. As controls, we used normal bladder tissue from five patients submitted to retropubic prostatectomy to treat benign prostatic hyperplasia. Extraction, cDNA and amplification were performed for 14 miRNAs (miR-100, -10a, -21, -205, -let7c, -143, -145, -221, -223, -15a, -16, -199a and -452) using specific kits, and RNU-43 and -48 were used as endogenous controls. Statistical tests were used to compare tumor size, multiplicity and episodes of recurrence with miRNAs expression profiles. Results There was a marginal correlation between multiplicity and miR-let7c over-expression. For all others miRNA no correlation between their expression and prognostic factors was found. Conclusion We did not find differences for miRNAs expression profiles associated with prognostic factors in tumor group studied. The majority of miRNAs are down-regulated, except miR-10a, over-expressed in most of cases, seeming to have increased levels in tumor with more unfavorable prognostic factors. More studies are needed in order to find a miRNA profile able to provide prognosis in pTa UC to be used in clinical practice. .
Subject(s)
Aged , Aged, 80 and over , Female , Humans , Male , Middle Aged , Carcinoma/genetics , MicroRNAs/analysis , Ureteral Neoplasms/genetics , Urinary Bladder Neoplasms/genetics , Analysis of Variance , Case-Control Studies , Carcinoma/pathology , Down-Regulation , Gene Expression , Gene Expression Profiling , Neoplasm Recurrence, Local/genetics , Neoplasm Recurrence, Local/pathology , Prognosis , Reference Values , Statistics, Nonparametric , Tumor Burden/genetics , Biomarkers, Tumor/analysis , Ureteral Neoplasms/pathology , Urinary Bladder Neoplasms/pathologyABSTRACT
OBJECTIVE: MicroRNAs are noncoding RNA molecules involved in the development and progression of tumors. We have found that miRNA-100 is underexpressed in metastatic prostate cancer compared to localized disease. Conversely higher levels of miR-100 are related to biochemical recurrence after surgery. This suggests that miR-100 may be a context-dependent miRNA, acting as oncogene or tumor suppressor miRNA. Our aim is to demonstrate the role of miR-100 in the control of predicted target genes in prostate cancer cell lines. METHODS: Cell lines DU145 and PC3 were transfected with miR-100, antimiR-100 and after 24 h and 48 h of exposure, qRT-PCR and western blot were performed for mTOR, FGFR3, THAP2, SMARCA5 and BAZ2A. RESULTS: There was reduction in mTOR (p = 0.025), THAP2 (p = 0.038), SMARCA5 (p = 0.001) and BAZ2A (p = 0.006) mRNA expression in DU145 cells after exposure to miR-100. In PC3 cells, mTOR expression was decreased by miR-100 (p = 0.01). There was a reduction in the expression levels of proteins encoded by studied genes, ranging from 34% to 69%. CONCLUSIONS: We demonstrate that miR-100 is a context-dependent miRNA controlling BAZ2, mTOR, FGFR3, SMARCA5 and THAP2 that might be involved in PC progression. The elucidation of the roles of miRNAs in tumors is important because they can be used as therapeutic targets in the future. .